All-natural, hygienic,
and effortless to use, Fertile-Focus is also backed by research. For women
who are trying to conceive, Fertile-Focus is 98%
accurate in aiding the prediction of fertile periods. The
accuracy claim is based on testing methodology (research on the saliva microscope
testing method of ovulation prediction).
Research
International Review of Medical Sciences
A New Test For Female Ovulation DiagnosisREPRINT Vol 6 - Nº 1, 1994 Edizioni
Universitarie Romane Fertility Tester G. GALATI*, E.TRAPANI, M.YACOUB, M.R. TOCCACELI,
G.M. GALATI,C. FIORELLI, F. BANDIERA, A. PAOLILLO // III Clinica Ostetrica e
Ginecologica, Università degli Studi di Roma "La Sapienza" (Direttore:
Prof. L. Marzetti)
Abstract
The Authors performed the saliva
test on nº328 women using the Saliva Tester. In nº48 cases the
test results revealed a fern-like crystallized structures as illustrated
in "Figure 3"below. In nº20 cases they performed a Transvaginal Ultrasonography
(T.V.U.S.). In nº16 cases (80%) they found an ovarian follicle greater
than 2 cm.To know the days of their menstrual cycle when fecundation is possible
has always been a demand felt by women and this both in the case when they
want to avoid it (1). Many methodologies exist that enable us to verify or
not the presence of a mature ovarian follicle or its happened explosion.
A very simple methodology was reached with observation and testing of the
Saliva Tester; results that may be easily and directly realized by any woman,
at any moment of the day, anywhere. The prospective advantages that descend
as to the knowledge of the useful days for a fecundation are such that they
led us to directly and correctly try this new test.
Material
and Method
It has been considered
the use of a mini-microscope, or "Saliva Fertility Tester", made up of a
small cylindrical body or eyepiece where we note an optical part with an
opposite small slide where a sample of saliva is placed. As soon as the sample
of saliva has dried, the small eyepiece body is put into a cylindrical container,
that leads down to a lighting system. By focusing the lens, a fern-like characteristic
crystallization is pointed out, in a very near correlation with the ovulation
period (Figure 3). Out of the fertile days there are no fern-like crystallization
structures seen, as pictures named "Figure 1" (during the non-fertile period)
and "Figure 2" (during the intermediate period) indicate. See image below.
We have taken into consideration
328 women between 15 and 50 years old, not using OCP who were placed under
our observation at the out-patients' departments of the Institute of Obstetrics
and Gynecology of the University "La Sapienza" in Rome. No selective principle
has been adopted. We have only invited the patient to leave a sample of saliva
on the slide of the mini-microscope, and afterwards age, day of the cycle
in which the patient was and length of her cycle were noted. 48 women, whose
sample of saliva gave the fern-like characteristically stratification were
invited to undergo an ultrasonography with transvaginal sound for the control
of the ovarium and of the presence or not of the pertinent follicle. 20 women
accepted our request, 28 women refused.
Results
Results are reported by Tables
1,2 and 3. In Table 1 we see nº48 women of nº328 who, as far as
anamnesis is concerned, are in the ovulation period; nº40 of these women
gave us the picture "Figure 3" type after test, nº40 gave us the picture "Figure
2" and nº248 the picture "Figure 1" type (Table 2). At the anamnesis,
nº 36 women were in the intermediate period; in all 36 cases of this
group, we have noted a picture "Figure 2" type. At the anamnesis, nº 244
women were in the non-fertile period and we have noted a picture "Figure
1" type in all the 244 cases. In the Table 3, are pointed out the nº20
cases who, at the saliva test, showed a picture "Figure 3" type and have
accepted to undergo sonographic control by means of transvaginal sound.
Table 1 - Number of women undergoing the saliva test, correlated
to the supposed fertile or non-fertile period of the cycle.
Day
of the cycle |
Non-fertile
period |
Intermediate
period |
Fertile
period |
Total |
N° of
women undergoing test |
244 |
36 |
48 |
328 |
Table 2 - Picture stressed by means of the saliva test.
Stressed picture |
Figure 1 |
Figure
2
|
Figure 3 | Total |
N° Cases |
248 |
40 |
40 |
328 |
Table 3 - Echographic
control of nº 20 women by means of picture Figure 3 type of the saliva
test.
Ovarium
Echographic Control
|
Follicle Absence |
Follicle < 1 cm |
Follicle > 2 cm |
N° cases
|
0 |
4 |
16 |
In 16 cases, equal to 80% of the total, an ovarian follicle has been stressed
having dimension over 2 cm, in 4 cases a follicle having diameter less
than 1 cm has been stressed.
References
1) Zondek B., Rozin S.: Cervical
mucus arborization: its use in determining of corpus luteum function.
Obstet. and Gynec., 3: 463, 1954.
2) Barbato M., Boerci M., et al.: Natural methods for fertility control.
New Trends Gynaec. Obstet., 2: 325, 1986.
3) Calamera J.C., Vilar O., Nicholson R., change in sialic acid concentration
in human saliva during the menstrual cycle. Int.Fertility 1986, 31; s.43-45
4) Folan J., Gosling J.P., Finn M.F., Fottrell P.F.; Solid Phase, Enzimoimmunoassay
of estrone in saliva, Clin.Chem., 1989, 35; s.569-571
5) Mancuso S., Van Look P.F.A.: Natural fertility regulation today. Intern.
J. Gynecol. Obstet., Suppl. I, 1989.
Conclusions
The Saliva Fertility Tester can
be used to determine the fertile period: the fertile cycle will be identified
with a continuous use, cycle after cycle. The Tester will also allow, by
repeating every month the tests, to obtain some significant indications about
the monthly cycle behavior and the hormonal situation of the woman. The Fertility
Tester can be used to avoid an undesired pregnancy: the comparison of the
figures given in Tables1 and 2 indicate the following: at the anamnesis n° 244
women were in the non-fertile period and we have noted a picture "Figure
1" type. In all 244 cases at the anamnesis n° 36 women were in the intermediate
period; in all 36 cases of this group, we have noted a picture "Figure 2" type.
In both cases we had a perfect coincidence between supposed fertile or infertile
days of the cycle and tests executed with "The Saliva Fertility Tester".
At the anamnesis 48 women were in the fertile period; in 40 of them we have
noted a picture "Figure 3" type. Such difference is due to the fact that
4 of them had a meal one hour before testing, thus invalidating the result,
and the other 4 may have not correctly performed the test. Therefore, even
if we assume that all these 8 women were in fact fertile (very unlikely),
the final result of such test is that ONLY
8 women out a panel of 328, i.e. less than 3%, resulted in false negative
(in fact fertile) at the saliva testing.
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